Clontech’s new Mag-Trypsin (TPCK-trypsin immobilized on magnetic beads) allows trypsin to be removed from a protein digestion mixture using a magnetic separator after the reaction is complete. TPCK treatment prior to immobilization inhibits chymotrypsin activity without any effect on trypsin. Without TPCK treatment, chymotrypsin catalyzes the hydrolysis of peptide bonds adjacent to the carboxyl group of the aromatic amino acids tryptophan, tyrosine, and phenylalanine.
Unlike agarose-linked immobilized trypsin, Mag-Trypsin does not require any centrifugation or use of columns. It provides a simple method for eliminating trypsin contamination after digesting protein samples to their peptide constituents in preparation for such techniques as mass spectroscopy or peptide enrichment.
Published in Drug Discovery & Development magazine: Vol. 10, No. 11, November, 2007, p.8.
Filed Under: Genomics/Proteomics