|Kidney section (mouse). Maximum projection of a stack of 65 single images. Green: Glomeruli and convoluted tubules (wheat germ agglutinin Alexa Fluor 488); blue: cell nuclei (DAPI).|
Intelligent Structured Illumination Microscopy from Leica Microsystems uses the proven OptiGrid technique. Working in harmony with Leica’s optics, the aperture diaphragm integrated with the fluorescence light path also enhances contrast. The automatic focus function of the product keeps the grid structure in focus, from UV to IR light.
Resolution and contrast are crucially important for fluorescence applications in widefield microscopy. Undesired haze effects that reduce contrast can be minimized by using the smart principle of structured illumination. The result: fluorescence images with excellent contrast, superb axial resolution and ultra-sharp 2D sections of the specimen.
A unique feature of this development is that the same OptiGrid module can be used both for upright and for inverted Leica research microscopes. Furthermore, there is no need to change the grid, as one optimized grid covers all magnifications from 10x to 100x, which offers convenience, avoids errors, and saves valuable time.
Filed Under: Drug Discovery