NanoSight introduced the NS500 system, which incorporates new hardware and software to deliver NanoSight’s growing capability in particle-by-particle characterization in an automated package. The first system is in use at the University of Oxford in the Nuffield Department of Obstetrics and Gynaecology of the John Radcliffe Hospital as part of a program supported by the Wellcome Foundation.
NanoSight’s technology, known as Nanoparticle Tracking Analysis (NTA), provides a high-resolution particle size distribution, and not by DLS (dynamic light scattering). NTA detects individual particles as small as 20 nm and, in real time, simultaneously tracks and sizes whole populations. The result is a particle size distribution that provides researchers with an over view of their samples showing everything in the whole range 20 to 1,000 nm. NTA also provides count and concentration, together with a unique view which validates these results.
The NS500 adds fluorescence capability, enabling the user to tune into individual particles, with sensitivity to detect individual quantum dots whilst eliminating background interference of other particles and media. Standard beads may be used to bind to single particles for optimum study.
The fluid handling capability of NS500 provides the user with auto sample presentation and in-situ cleaning. A routine process cleans the cell by purging, flushing, and loading samples through user-customizable software. Dilution may also be optimized and controlled in this way. A motorized focus function helps home in on the particles under study. This is augmented with an indexed motorized stage, controlled through the software and providing repeatability in positioning. The temperature control of the cell offers a broad range (15?C to 55?C) and programmable temperature cycling, with rapid attainment of set-point facilitating faster sample measurement and turnaround.
Filed Under: Drug Discovery