Velocity11 released a technical note describing an automated Polymerise Chain Reaction (PCR) workstation based upon a combination of their Bravo liquid handler, BenchCel plate handling system, PlateLoc thermal microplate sealer and a bulk reagent dispenser.
The automated workstation is shown to provide a reliable, efficient solution for processing plates in batches or one at a time through the necessary stages of PCR plate preparation. Using the described protocol up to 50 diluted replicate plates with Master Mix and Exon additions can be run without user intervention.
The on-deck accessories for the Bravo liquid handler are shown to provide the temperature control and sonicating wash to decontaminate tips that are necessary capabilities for working with DNA and other sensitive materials. Velocity 11’s VWorks automation control software was used to provide a single, simple user interface to control all critical task parameters.
The combination of Velocity11’s Bravo liquid handler, BenchCel plate handling system, PlateLoc thermal microplate sealer, and a bulk reagent dispenser is shown to allow users to run the PCR preparation with high throughput and maximum walk-away time.
Filed Under: Drug Discovery