Invitrogen Corporation introduced the SequalPrep System; a complete sample enrichment and normalization solution for next generation sequencing technologies that consists of the SequalPrep Long PCR Kit and the SequalPrep Normalization Plate (96) Kit.
One of the most significant applications of next generation sequencing technologies is the sequencing of specific DNA regions to identify rare genetic mutations that might contribute to disease development. To accomplish this, scientists select DNA sections associated with a disease state and amplify them using PCR. The resulting sections are put into sequencing processing where they are assembled to determine the exact nucleotide sequence for further analysis.
The SequalPrep Long PCR Kit allows researchers to reduce the number of enrichment reactions required by providing long range PCR with high amplification success of up to 20 kilobases and little or no optimization.
In order to achieve higher depth of coverage across the entire DNA region, researchers must ensure the starting material for each DNA section is relatively equivalent or normalized. The SequalPrep Normalization Plate (96) Kit reduces labor and errors by providing one-step PCR product purification and normalization.
This article was published in Drug Discovery & Development magazine: Vol. 11, No. 7, July, 2008, pp. 23.
Filed Under: Genomics/Proteomics