Drug Discovery and Development

  • Home Drug Discovery and Development
  • Drug Discovery
  • Genomics/Proteomics
  • Oncology
  • Neurological Disease
  • Infectious Disease
  • R&D 100 Awards
  • Pharma 50
    • 2022 Pharma 50
    • 2021 Pharma 50

Scientists Create Primate Embryonic Stem Cells

By Drug Discovery Trends Editor | November 14, 2007

Researchers at Oregon Health & Science University’s (OHSU) Oregon National Primate Research Center have successfully derived embryonic stem cells by reprogramming of genetic material from skin cells while studying rhesus macaque monkeys. The breakthrough follows several previously unsuccessful attempts by the OHSU-based team and other scientific teams worldwide. The results of the work were released by the scientific journal Nature. The work will also be published in  the journal.

Prior to the OHSU team’s recent success in a species closely related to humans, scientists worldwide have isolated stem cells only in mice using a technique called somatic cell nuclear transfer. The method involves transplanting the nucleus of the cell, containing an individual’s DNA, to an egg cell which has had its genetic material removed. For various reasons and despite numerous attempts, previous efforts to use the SCNT technology to clone stem cells in primates have failed repeatedly.

“Many scientists believe that embryonic stem cells hold great promise for treating a variety of diseases including Parkinson’s disease, multiple sclerosis, cardiac disease and spinal cord injuries,” explained Shoukhrat Mitalipov, PhD, director of the OHSU-based research team and an assistant scientist in the Division of Reproductive Sciences at ONPRC, the Oregon Stem Cell Center and the department of Obstetrics and Gynecology of Oregon Health & Science University. Don Wolf, PhD, of the primate center also played a significant role in the research.

The reason why Mitalipov’s team was successful when so many other previous attempts were not, lies in the method for identifying and extracting the nuclei of the eggs being used. Prior attempts resulted in damaged eggs due to the difficulties involved in removing the nucleus. This means that the eggs were not fully functional and failed to divide and develop.

To conduct the research, researchers obtained skin cells from a nine-year-old male rhesus macaque monkey at the Oregon National Primate Research Center. The researchers then used specialized imaging software called, Oosight Spindle Imaging System, to spot and remove the nuclear material attached to the egg’s spindle fibers. The nuclei of skin cells were then inserted into nucleus-free eggs. Using this technique, two embryonic stem cell lines – groups of cells that can grow indefinitely and differentiate into any cells of the body- were successfully developed. The genetic material (DNA) of cell lines was then matched to DNA from the male donor male monkey to ensure that they were a direct clone.

Release date: November 14, 2007

Source: Oregon Health & Science University


Filed Under: Genomics/Proteomics

 

Related Articles Read More >

DNA Script SYNTAX
DNA Script raises $165M Series C for DNA printing platform
DNA Script SYNTAX
DNA Script launches a benchtop DNA printer
DNA
Unraveling the promise of genetics for treating progressive illness 
Alzheimer's disease
How to build a foundation for the next phase of Alzheimer’s disease research

MEDTECH 100 INDEX

Medtech 100 logo
Market Summary > Current Price
The MedTech 100 is a financial index calculated using the BIG100 companies covered in Medical Design and Outsourcing.

Need Drug Discovery news in a minute?

We Deliver!
Drug Discovery & Development Enewsletters get you caught up on all the mission critical news you need. Sign up today.
Enews Signup
Drug Discovery and Development
  • MASSDEVICE
  • DEVICETALKS
  • Medical Design & Outsourcing
  • MEDICAL TUBING + EXTRUSION
  • MEDTECH 100
  • Medical Design Sourcing
  • Subscribe to our Free E-Newsletter
  • Contact Us
  • About Us
  • Advertise With Us
  • R&D World
  • Drug Delivery Business News
  • Pharmaceutical Processing World

Copyright © 2022 WTWH Media LLC. All Rights Reserved. The material on this site may not be reproduced, distributed, transmitted, cached or otherwise used, except with the prior written permission of WTWH Media
Privacy Policy | Advertising | About Us

Search Drug Discovery & Development

  • Home Drug Discovery and Development
  • Drug Discovery
  • Genomics/Proteomics
  • Oncology
  • Neurological Disease
  • Infectious Disease
  • R&D 100 Awards
  • Pharma 50
    • 2022 Pharma 50
    • 2021 Pharma 50