Scientists Create Primate Embryonic Stem Cells - Drug Discovery and Development

Researchers at Oregon Health & Science University’s (OHSU) Oregon National Primate Research Center have successfully derived embryonic stem cells by reprogramming of genetic material from skin cells while studying rhesus macaque monkeys. The breakthrough follows several previously unsuccessful attempts by the OHSU-based team and other scientific teams worldwide. The results of the work were released by the scientific journal Nature. The work will also be published in the journal.

Prior to the OHSU team’s recent success in a species closely related to humans, scientists worldwide have isolated stem cells only in mice using a technique called somatic cell nuclear transfer. The method involves transplanting the nucleus of the cell, containing an individual’s DNA, to an egg cell which has had its genetic material removed. For various reasons and despite numerous attempts, previous efforts to use the SCNT technology to clone stem cells in primates have failed repeatedly.

“Many scientists believe that embryonic stem cells hold great promise for treating a variety of diseases including Parkinson’s disease, multiple sclerosis, cardiac disease and spinal cord injuries,” explained Shoukhrat Mitalipov, PhD, director of the OHSU-based research team and an assistant scientist in the Division of Reproductive Sciences at ONPRC, the Oregon Stem Cell Center and the department of Obstetrics and Gynecology of Oregon Health & Science University. Don Wolf, PhD, of the primate center also played a significant role in the research.

The reason why Mitalipov’s team was successful when so many other previous attempts were not, lies in the method for identifying and extracting the nuclei of the eggs being used. Prior attempts resulted in damaged eggs due to the difficulties involved in removing the nucleus. This means that the eggs were not fully functional and failed to divide and develop.

To conduct the research, researchers obtained skin cells from a nine-year-old male rhesus macaque monkey at the Oregon National Primate Research Center. The researchers then used specialized imaging software called, Oosight Spindle Imaging System, to spot and remove the nuclear material attached to the egg’s spindle fibers. The nuclei of skin cells were then inserted into nucleus-free eggs. Using this technique, two embryonic stem cell lines – groups of cells that can grow indefinitely and differentiate into any cells of the body- were successfully developed. The genetic material (DNA) of cell lines was then matched to DNA from the male donor male monkey to ensure that they were a direct clone.

Release date: November 14, 2007

Source: Oregon Health & Science University