Bio-Rad Laboratories, Inc. has published a technical note (bulletin 5514A) that compares Bio-Rad’s Profinia protein purification system with the ÄKTAprime system from GE Healthcare, for the purification and desalting of His-tagged proteins. Like the Profinia system, the ÄKTAprime system offers preprogrammed methods,pre-formulated buffers, and chromatography cartridges. The comparison was focused on ease-of use, reliability, and overall performance of the two systems.
The Profinia system is fully automated and requires no user handling or intervention between application of sample, affinity purification, and desalting of the purified protein. In addition, Profinia IMAC purification reagents, including solutions and cartridges, require no advanced preparation, while the ÄKTAprime HisTrap reagent kits provide buffer concentrates that must be formulated and diluted by the user, according to Bio-Rad. While protein yields and purities generated by both systems were nearly equivalent, the Profinia system saved over one hour from each affinity purification.
Bulletin 5514A is available either from a local Bio-Rad sales office or can be downloaded from the Bio-Rad Web site.
Filed Under: Drug Discovery