Drug Discovery and Development

  • Home Drug Discovery and Development
  • Drug Discovery
  • Genomics/Proteomics
  • Oncology
  • Neurological Disease
  • Infectious Disease
  • R&D 100 Awards
  • Pharma 50
    • 2022 Pharma 50
    • 2021 Pharma 50

New Platform for Roundworms Could Speed Up Drug Delivery

By University of Texas at Austin | October 12, 2016

Researchers in the Cockrell School of Engineering at The University of Texas at Austin have developed the first large-scale in vivo drug discovery platform using a whole animal model that could speed up scientific research and more accurately assess the effectiveness of new drugs in the treatment of neurodegenerative diseases.

Professor Adela Ben-Yakar and her team of postdocs and graduate students—Sudip Mondal, Evan Hegarty, Christopher Martin, Sertan Kutal Gökçe and Navid Ghorashian—describe their new platform in a study published in the journal Nature Communications on Oct. 11.

The team believes their platform will allow researchers and pharmaceutical companies to undertake image-based analysis of thousands of live, 1-millimeter-long roundworms, called Caenorhabditis elegans (C. elegans), simultaneously and at the speed and cost of in vitro cell-based platforms.

Ben-Yakar, a researcher in the Departments of Mechanical and Biomedical Engineering, led the design of the new platform, which takes the form of a cellphone-size chip that can immobilize and analyze small animal models using a high-powered microscope. The platform is similar to screening platforms for cells, but it can screen a whole organism made of multiple cells by immobilizing thousands of them inside micro-traps to prevent their natural movement. In addition, the platform can automatically understand data using image analysis software, which was also developed by the researchers. This information can help determine whether a drug is effectively treating a disease in the roundworms.

The researchers’ microfluidic device is designed to simultaneously image approximately 3,600 roundworms at high resolutions and high speeds, ranging from 12 to 16 minutes. Today’s conventional platforms can image only a few animals within such a short time. The device also has the ability to assess the efficacy of many different drug compounds at once. Whereas other microfluidic platforms used today are capable of analyzing only one drug compound at a time, the researchers’ new platform can assess 96.

The roundworms, which are desirable in the study of neurodegenerative diseases because they have full nervous systems and a diverse group of neurons, are also the first multicellular organism to have a completely sequenced genome. Approximately one-third of C. elegans’ genes are the same as disease-causing genes in humans.

“The C. elegans are thousands of times bigger than cells, so now that we have developed a way to capture and immobilize so many of them so quickly, we can determine much more information about the efficacy of drugs in a whole organism rather than the limited information that is derived when we used isolated individual cells,” Ben-Yakar said.

What sets the chip apart from existing platforms is that it is capable of high-throughput screening, the study of thousands to hundreds of thousands of compounds in a rapid and very high-resolution manner, and high-content fluorescence screening, which allows for a faster yet more in-depth analysis of the animals’ phenotype.

Made of a flexible polymer, the microfluidic chip contains 96 wells for receiving drug-treated animals. Underneath each well are special microchannels that can trap 40 roundworms. A tapered design squeezes the C. elegans into microchannels in a flat orientation that can be more easily imaged. The microfluidic chip then relies on a gasket system that applies pressure to the chambers to immobilize the roundworms.

“This proof-of-principle study opens the door to using this technology to identify and develop treatments for some of the most intractable human diseases,” said Anne Hart, a professor of neuroscience at Brown University who has followed the development of the chip.

To demonstrate the efficiency of their patent-pending device, the team analyzed approximately 1,000 drugs approved by the Food and Drug Administration aimed at treating the protein aggregation found in various neurodegenerative diseases, including Huntington’s disease. In doing so, they were surprised to find a potential drug that is particularly effective at treating Huntington’s.

“Four of the drugs we tested were effective at treating a protein aggregation model that is directly related to Huntington’s, and one of these drugs became even more effective at increased doses without causing toxicity,” Ben-Yakar said.

Although the microfluidic device is a prototype, Ben-Yakar’s startup company, Newormics, is working to develop a design for a commercial product that could be cheaply and easily manufactured.

Video caption: Texas Engineers have developed the first large-scale in vivo drug discovery platform using C. elegans—a transparent roundworm that is used in the study of neurodegenerative diseases, including Parkinson’s, Huntington’s and Alzheimer’s. (Credit: Cockrell School of Engineering)

To view the full press release, click here. 

Follow us on Twitter and Facebook for updates on the latest pharmaceutical and biopharmaceutical manufacturing news! 


Filed Under: Drug Discovery

 

Related Articles Read More >

Takeda Pharmaceutical in the Drug Discovery & Development Pharma 50
Takeda’s Takhzyro fares well in pediatric hereditary angioedema study
Novartis in the Pharma 50
Novartis to cut up to 8,000 positions
Gilead Sciences in the Drug Discovery & Development Pharma 50
Gilead resubmits application to FDA for twice-yearly HIV drug lenacapavir
George Floyd mural
How the pandemic and George Floyd made clinical trial diversity a priority

MEDTECH 100 INDEX

Medtech 100 logo
Market Summary > Current Price
The MedTech 100 is a financial index calculated using the BIG100 companies covered in Medical Design and Outsourcing.

Need Drug Discovery news in a minute?

We Deliver!
Drug Discovery & Development Enewsletters get you caught up on all the mission critical news you need. Sign up today.
Enews Signup
Drug Discovery and Development
  • MASSDEVICE
  • DEVICETALKS
  • Medical Design & Outsourcing
  • MEDICAL TUBING + EXTRUSION
  • MEDTECH 100
  • Medical Design Sourcing
  • Subscribe to our Free E-Newsletter
  • Contact Us
  • About Us
  • Advertise With Us
  • R&D World
  • Drug Delivery Business News
  • Pharmaceutical Processing World

Copyright © 2022 WTWH Media LLC. All Rights Reserved. The material on this site may not be reproduced, distributed, transmitted, cached or otherwise used, except with the prior written permission of WTWH Media
Privacy Policy | Advertising | About Us

Search Drug Discovery & Development

  • Home Drug Discovery and Development
  • Drug Discovery
  • Genomics/Proteomics
  • Oncology
  • Neurological Disease
  • Infectious Disease
  • R&D 100 Awards
  • Pharma 50
    • 2022 Pharma 50
    • 2021 Pharma 50