Dealing with membrane proteins in lipidic mesophases is not an easy task. Until now the sample preparation has been a very tedious and time-consuming task.
Zinsser Analytic has developed ProCrys Meso, the new liquid handling platform for the preparation of 96 in-meso (or cubic phase) proteins. It offers accurate and precise dispensing of the protein and the screening solution and also can be used to prepare your own screening solutions on the workbench instead of using the more expensive commercial kits. An integrated module ensures the ideal humidity conditions for the process. Clever tools have been incorporated to set up a run more easily, including precise and reproducible dispensing of the mesophase.
ProCrys Meso is based on a 1 meter liquid handling platform with 2 pipetting arms. The first pipetting arm is equipped with pipetting probes for the preparation of the screening solutions and its distribution to the crystallization plate. The second arm is used for the transfer of the nanoliter volumes of the protein solution. The protein and the screening solution, as well as the crystallization plate are placed on the workbench.
The WinProCrys software allows maximum flexibility of method development. Work lists can be imported from Excel® or the customer’s LIMS system.
ProCrys Meso not only increases the throughput and the reproducibility of the experiments, it also reduces costs.
Filed Under: Drug Discovery